![]() ![]() All the isolates presented Extraintestinal pathogenic E. We isolated strains of O25b-ST131- fimH22 harboring gene bla CTX-M-8 (3 clones), ST117 harboring gene bla SHV-12, and a novel ST11350 (clonal complex 349) harboring genes bla CTX-M-55 and fosA3. We identified ESBL-EC isolates in 2.4% (5/204) of the tested seabirds, all in magnificent frigatebirds ( Fregata magnificens). Herein we surveyed ESBL-EC in 204 cloacal swabs from six nonsynanthropic seabird species at the pristine Rocas Atoll, Brazil. Remote locations represent a unique opportunity to evaluate the occurrence, dissemination and epidemiology of ARB in the environment. Wild birds have been commonly used as environmental bioindicators of ESBL-EC. Among antimicrobial resistant bacteria (ARB), extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli (ESBL-EC) represent major challenges to human healthcare. coli.Īntimicrobial resistance is among the most serious public health threats of the 21st century, with great impact in terms of One Health. The results of the current study may be helpful for the physicians the better management of the diseases caused by E. coli multi drugs resistant making it a causative agent for many diseases in human beings. The over-use and misuse of antimicrobial agents has made pathogenic E. To prevent and treat pathogenic diseases, the use of antimicrobial agents in animal husbandry are of utmost concern. The PCR products were sequenced by Next Generation Sequencing (NGS) and the results revealed mutations in AAC gene (M120T and R197T) and CTX-M15 (A85V, N122D, A148S and G247D). The isolates showed different antibiotic resistance genes OXA-1, CTX-M15, blaTEM, QnrS, TetA, AAC, AAD, sul1 and sul2 which is the molecular explanations of their antibiotic resistance pattern. The results were statistically significant having value P ≤ 0.05 in ANOVA. The isolates were resistant to TE, NOR and NA while were sensitive to MEM, TZP and FOS. coli among the collected chicken samples. A total of 174 samples were positive for E. The isolates were identified by Gram staining, API strips and through PCR (Universal Stress Protein). The current study was therefore aimed at identification of Escherichia coli, a common pathogen causing food borne infections, in chicken samples (n = 400) collected from three districts of KhyberPukhtunkhwa Peshawar, Kohat and Nowshera. But poultry meat can be contaminated by pathogenic bacteria which are responsible for food borne infections. In human diet, poultry meat is an important component due to the presence of vitamins, proteins and minerals. This study provides new data that highlight the importance of multidisciplinary and uncultured study in order to describe the spreading of antimicrobial resistance and related contamination in the environment. Of the antimicrobials investigated, the abundance of mcr-1 gene suggests the importance of monitoring the wildlife in order to detect the emerging resistance genes contamination in environment. Additionally, positive correlations between antibiotic resistance genes and microbial taxa (e.g., Tet genes correlated with Firmicutes and Patescibacteria) were described. Indeed, it was mostly characterized by phyla Firmicutes in bovine, Bacteroidota in chamois and Proteobacteria in red deer. The microbial community composition was found to be different based on the species and land use of animals under study. Samples were screened for commonly used in farms under study or critically important antimicrobial resistance genes ( aadA2, TetA, TetB, TetK, TetM, mcr-1). The populations under study were selected based on ecological data useful to define sympatric and non-sympatric populations. ![]() In total, 88 fecal samples were recovered from Apennine chamois, red deer, goat, cattle and sheep, and were collected in pools. This study aimed to provide new insights about antimicrobial resistance genes abundance and microbial communities of wild and domestic ruminants in wildlife-livestock interface.
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